4NT9
Crystal structure of an L,D-carboxypeptidase DacB from Streptococcus pneumonia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.110, 68.080, 80.350 |
| Unit cell angles | 90.00, 108.41, 90.00 |
Refinement procedure
| Resolution | 41.469 - 1.705 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.375 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.705 | 1.710 |
| Number of reflections | 58226 | |
| Completeness [%] | 97.9 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1M Tris-Cl, pH 8.5, 30% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
