4NT8

Formyl-methionine-alanine complex structure of peptide deformylase from Xanthomoonas oryzae pv. oryzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-22
Detector	ADSC QUANTUM 210
Wavelength(s)	0.99999
Spacegroup name	P 61 2 2
Unit cell lengths	58.584, 58.584, 266.188
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.350 - 2.200
R-factor	0.19244
Rwork	0.190
R-free	0.23547
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fr8
RMSD bond length	0.018
RMSD bond angle	1.856
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (6.4.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	16778
Completeness [%]	99.6	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	287	0.05M cadmium sulfate, 0.1M HEPES pH 7.5, 2.0M sodium acetate trihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 287K