4NT6
HLA-C*0801 Crystal Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-09-02 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.401, 75.687, 61.880 |
| Unit cell angles | 90.00, 120.29, 90.00 |
Refinement procedure
| Resolution | 55.460 - 1.840 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qqd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.597 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.460 | 1.880 |
| High resolution limit [Å] | 1.790 | 1.840 |
| Number of reflections | 35257 | |
| <I/σ(I)> | 19.2 | 3.3 |
| Completeness [%] | 66.6 | 89.9 |
| Redundancy | 12.65 | 2.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.05M CALCIUM CHLORIDE DIHYDRATE, 0.1M BIS-TRIS, 30%(V/V) POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
