4NSX
Crystal Structure of the Utp21 tandem WD Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.493, 63.540, 82.549 |
| Unit cell angles | 110.95, 95.83, 97.93 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.22420 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.834 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.142 | 0.478 |
| Number of reflections | 37483 | |
| <I/σ(I)> | 13.723 | 2.826 |
| Completeness [%] | 97.9 | 97.3 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 293 | 15% PEG3350, 0.15M sodium citrate, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
