4NSW
Crystal structure of the BAR-PH domain of ACAP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.460, 59.720, 168.950 |
Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
Resolution | 29.850 - 2.200 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.26600 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.459 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.850 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.082 | 0.418 |
Number of reflections | 41826 | |
<I/σ(I)> | 21.3 | 3.96 |
Completeness [%] | 96.9 | 95.6 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2M AMMONIUM CITRATE, 10% PEG3350, 6.0MM OCTYL BETA-THIOGLUCOPYRANOSIDE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2M AMMONIUM CITRATE, 10% PEG3350, 6.0MM OCTYL BETA-THIOGLUCOPYRANOSIDE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |