4NSS
A structural and functional investigation of a novel protein from Mycobacterium smegmatis implicated in mycobacterial macrophage survivability
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95369 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 50.050, 50.050, 205.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.420 - 2.400 |
R-factor | 0.1904 |
Rwork | 0.189 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.050 | 2.670 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 10787 | |
Completeness [%] | 98.7 | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | 1.42M Lithium Sulfate, 0.1M Tris HCl pH 7.7, 0.1M sodium citrate tribasic dihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |