4NSP
Crystal structure of human ENDOV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Nova high-flux-micro-focus sealed tube |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2013-09-24 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.750, 48.713, 108.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.755 - 2.300 |
| Rwork | 0.210 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hd0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.889 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.760 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.107 | 0.349 |
| Number of reflections | 9828 | |
| <I/σ(I)> | 9.6 | 3.5 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | 0.2M sodium formate, 0.1 M Tris-HCl PH7.5,19%PEG3350, EVAPORATION, temperature 298K |
