Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

4NSH

Carboplatin binding to HEWL in 0.2M NH4SO4, 0.1M NaAc in 25% PEG 4000 at pH 4.6

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsBRUKER AXS MICROSTAR-H
Temperature [K]100
Detector technologyCCD
Collection date2013-07-29
DetectorAPEX II CCD
Wavelength(s)1.5418
Spacegroup nameP 43 21 2
Unit cell lengths77.085, 77.085, 37.143
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.710 - 2.100
R-factor0.19934
Rwork0.196
R-free0.26188
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2w1y
RMSD bond length0.008
RMSD bond angle1.151
Data reduction softwareSAINT
Phasing softwarePHASER
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.7102.130
High resolution limit [Å]2.1002.100
Rmerge0.1260.499
Number of reflections6593
<I/σ(I)>19.22.2
Completeness [%]94.699.7
Redundancy20.85.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.629820mg of HEWL (0.6mM) was dissolved in 1ml distilled water. 1.4mg of carboplatin (1.8mM) was added with 75 l DMSO. 2 l of this mixed with 2 l resevoir soultion 0.2M NH4SO4, 0.1M NaAc in 25% PEG 4000 at pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

226707

PDB entries from 2024-10-30

PDB statisticsPDBj update infoContact PDBjnumon