4NRZ
Crystal Structure of HIV-1 Neutralizing Antibody m66.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-02-24 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.160, 118.873, 80.033 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 30.190 - 2.420 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.173 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.177 | 0.315 |
| Number of reflections | 35882 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 85.7 | 50.7 |
| Redundancy | 4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 11% polyethylene glycol 8000, 0.05 M zinc acetate, 0.05 M sodium cacaodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
