4NRS
Crystal Structure of Glycoside Hydrolase Family 5 Mannosidase (E202A mutant) from Rhizomucor miehei in complex with mannobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-01-01 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.622, 175.847, 56.295 |
Unit cell angles | 90.00, 104.34, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.570 |
R-factor | 0.19792 |
Rwork | 0.195 |
R-free | 0.24252 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.587 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.920 | 2.640 |
High resolution limit [Å] | 2.570 | 2.600 |
Rmerge | 0.099 | 0.330 |
Number of reflections | 27497 | |
<I/σ(I)> | 11.9 | 2.36 |
Completeness [%] | 96.8 | 88.3 |
Redundancy | 3.4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% mannobiose, 16% PEG 4000 (w/v), 200mM GdnHCl, 100mM HEPES buffer, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |