4NRL
Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2013-08-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872A |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 83.610, 128.400, 211.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.487 - 2.720 |
R-factor | 0.1961 |
Rwork | 0.194 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.932 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: dev_1452)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.910 | 2.870 |
High resolution limit [Å] | 2.720 | 2.720 |
Rmerge | 0.118 | 0.491 |
Number of reflections | 59313 | |
<I/σ(I)> | 13.7 | 4 |
Completeness [%] | 95.9 | 87.1 |
Redundancy | 6.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.1 M di-Sodium succinate 15% w/v PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |