4NRJ

Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2013-08-09
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97872
Spacegroup name	P 21 2 21
Unit cell lengths	83.690, 128.600, 211.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.182 - 2.530
R-factor	0.1883
Rwork	0.187
R-free	0.22150
Structure solution method	MIR
RMSD bond length	0.003
RMSD bond angle	0.877
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: dev_1452))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.630	2.670
High resolution limit [Å]	2.530	2.530
Rmerge	0.114	0.483
Number of reflections	75220
<I/σ(I)>	13.1	4.4
Completeness [%]	97.9	93.9
Redundancy	7.4	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	0.1 M Di-sodium succinate 15% w/v PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K