4NRH
CopN-Scc3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.571, 96.183, 94.472 |
| Unit cell angles | 90.00, 92.27, 90.00 |
Refinement procedure
| Resolution | 47.199 - 2.200 |
| R-factor | 0.1853 |
| Rwork | 0.184 |
| R-free | 0.22670 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8.4_1496) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.059 | 0.553 |
| Number of reflections | 50257 | |
| <I/σ(I)> | 24.2 | 1.6 |
| Completeness [%] | 90.0 | 53.7 |
| Redundancy | 7.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 200mM Na/K Tartate, 18-22 % PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
