4NRG
Crystal Structure of a human Mms2/Ubc13 D118G mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-02-04 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.272, 73.616, 92.093 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.136 - 1.950 |
R-factor | 0.1799 |
Rwork | 0.178 |
R-free | 0.21080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7d |
RMSD bond length | 0.005 |
RMSD bond angle | 0.860 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
Rmerge | 0.090 | 0.041 | 0.439 |
Number of reflections | 21609 | ||
<I/σ(I)> | 15.7 | ||
Completeness [%] | 95.3 | 97.1 | 89.3 |
Redundancy | 4.2 | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 20% PEG 8000, 0.1 M sodium citrate, pH 6.8, vapor diffusion, sitting drop, temperature 298K |