4NR9
Crystal Structure of the bromodomain of human BAZ2B in complex with acetylated lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.439, 96.754, 57.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.500 - 1.980 |
| R-factor | 0.19296 |
| Rwork | 0.191 |
| R-free | 0.23335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g0l |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.633 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.670 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.084 | 0.716 |
| Number of reflections | 16407 | |
| <I/σ(I)> | 10.9 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277.15 | 30% low MW PEG Smears, 0.1M MES pH 6.7, 15%(v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
