4NQY

The reduced form of MJ0499

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 41
Unit cell lengths	117.010, 117.010, 84.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.360 - 2.600
R-factor	0.206
Rwork	0.204
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kp1
RMSD bond length	0.006
RMSD bond angle	1.277
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.910	2.740
High resolution limit [Å]	2.600	2.600
Number of reflections	34280
Completeness [%]	96.8	99.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.2	293	0.1M Tis, 20% PEG 400, 0.5% LDAO(n-Dodecyl-N,N,dimethylamine-N-oxide), pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K