4NQI

Structure of the N-terminal I-BAR domain (1-259) of D.Discoideum IBARa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	59.100, 60.210, 147.620
Unit cell angles	90.00, 100.38, 90.00

Refinement procedure

Resolution	48.401 - 2.210
R-factor	0.1962
Rwork	0.194
R-free	0.23030
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.210	2.210
Number of reflections	99218
<I/σ(I)>	10.21	1.88
Completeness [%]	98.5	85.3
Redundancy		2.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20 % PEG 3350, 0.25M ammonium acetate, 15% v/v glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K