4NQI
Structure of the N-terminal I-BAR domain (1-259) of D.Discoideum IBARa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9796 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.100, 60.210, 147.620 |
Unit cell angles | 90.00, 100.38, 90.00 |
Refinement procedure
Resolution | 48.401 - 2.210 |
R-factor | 0.1962 |
Rwork | 0.194 |
R-free | 0.23030 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.766 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 99218 | |
<I/σ(I)> | 10.21 | 1.88 |
Completeness [%] | 98.5 | 85.3 |
Redundancy | 2.48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20 % PEG 3350, 0.25M ammonium acetate, 15% v/v glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |