4NQG

Crystal Structure of Ca(2+)-regulated photoprotein mitrocomin from Jellyfish Mitrocoma cellularia at 1.3 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	48.898, 69.682, 49.929
Unit cell angles	90.00, 90.03, 90.00

Refinement procedure

Resolution	40.586 - 1.301
R-factor	0.1624
Rwork	0.161
R-free	0.19040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qv0
RMSD bond length	0.013
RMSD bond angle	1.407
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.350
High resolution limit [Å]	1.300	1.300
Number of reflections	54457
Completeness [%]	66.4	2.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	1.4M sodium citrate tribasic dihydrate solution, 0.1M HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K