4NQ3
Crystal structure of cyanuic acid hydrolase from A. caulinodans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 97 |
| Detector technology | CCD |
| Collection date | 2012-06-15 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.97864 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 237.866, 237.866, 105.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.794 - 2.702 |
| R-factor | 0.1611 |
| Rwork | 0.159 |
| R-free | 0.19130 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.188 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 42770 | |
| Completeness [%] | 98.4 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1M MgSO4, Tris, pH 7.0 - 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
