4NPB
The crystal structure of thiol:disulfide interchange protein DsbC from Yersinia pestis CO92
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 90.894, 141.335, 41.153 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.505 - 2.147 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23520 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.068 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.100 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.102 | 0.664 |
| Number of reflections | 29556 | |
| <I/σ(I)> | 28.8 | 2.57 |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 289 | 0.056M Sodium Phosphate monobasic monohydrate, 1.344M potassium phosphate dibasic, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
