4NP8
Structure of an amyloid forming peptide VQIVYK from the second repeat region of tau (alternate polymorph)
Replaces: 3FQPExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 28.635, 4.880, 35.807 |
Unit cell angles | 90.00, 110.47, 90.00 |
Refinement procedure
Resolution | 16.780 - 1.510 |
R-factor | 0.1791 |
Rwork | 0.177 |
R-free | 0.19590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2on9 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.399 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 1.620 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.160 | 0.376 |
Number of reflections | 825 | |
<I/σ(I)> | 8.1 | 4.7 |
Completeness [%] | 94.4 | 82.1 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | reservoir contained 14% iso-Propanol, 0.07M HEPES-Na pH 7.5, 0.14M Sodium Citrate, and 30% Glycerol, vapor diffusion, hanging drop, temperature 298K |