4NP1
NITROPHORIN 1 COMPLEX WITH NITRIC OXIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1997-11-17 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.410, 74.290, 66.120 |
| Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.30000 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1np1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | AGROVATA/ROTAVATA |
| Data scaling software | Agrovata |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.200 |
| Number of reflections | 18071 | |
| <I/σ(I)> | 9.2 | 2.5 |
| Completeness [%] | 94.5 | 87.7 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.8 M AMMONIUM PHOSPHATE, 0.1 M TRIS.HCL, PH 7.5, ROOM TEMPERATURE |
