4NOX

Structure of the nine-bladed beta-propeller of eIF3b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.826580
Spacegroup name	P 43 21 2
Unit cell lengths	108.380, 108.380, 172.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.469 - 2.722
R-factor	0.2069
Rwork	0.206
R-free	0.22980
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.806
Data scaling software	XSCALE
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.469		2.820
High resolution limit [Å]	2.720	50.000	2.720
Rmerge	0.057		0.615
Rmeas			0.653
Number of reflections	28259		2828
<I/σ(I)>	25.67		3.48
Completeness [%]	99.9		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1
1