4NOO
Molecular mechanism for self-protection against type VI secretion system in Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.502, 116.034, 68.225 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 29.500 - 2.300 |
| R-factor | 0.18868 |
| Rwork | 0.186 |
| R-free | 0.23441 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.278 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.422 |
| Number of reflections | 34369 | |
| <I/σ(I)> | 28 | 4.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 15% (m/v) PEG8000, 0.1M Sodium Cacodylate pH5.6, 0.2M Mg acetate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
