4NOG
Crystal structure of a putative ornithine aminotransferase from Toxoplasma gondii ME49 in complex with pyrodoxal-5'-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-31 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 |
Unit cell lengths | 56.190, 61.328, 63.678 |
Unit cell angles | 100.58, 93.23, 107.74 |
Refinement procedure
Resolution | 62.140 - 1.200 |
R-factor | 0.13481 |
Rwork | 0.133 |
R-free | 0.16494 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lg0 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.927 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.140 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.043 | 0.370 |
Number of reflections | 224574 | |
<I/σ(I)> | 11.2 | 2 |
Completeness [%] | 91.0 | 91 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium Acetate, 0.1 M Bis-Tris, 35% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |