4NOB

Crystal structure of the 1st Ig domain from mouse Polymeric Immunoglobulin receptor [PSI-NYSGRC-006220]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0750
Spacegroup name	P 21 21 21
Unit cell lengths	40.275, 46.803, 62.062
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.370 - 1.510
R-factor	0.1625
Rwork	0.161
R-free	0.18910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xed
RMSD bond length	0.023
RMSD bond angle	2.214
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.540
High resolution limit [Å]	1.510	4.100	1.510
Rmerge	0.077	0.068	0.353
Number of reflections	18980
<I/σ(I)>	8.1
Completeness [%]	99.4	99.9	93.3
Redundancy	11.9	11.5	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	Protein (20 mM Hepes, pH 7.5, 150 mM NaCl, 5% glycerol), Reservoir (0.2M MgCl2, 0.1M Bis-Tris-HCl, 25% (v/v) PEG 3350), Cryoprotection (33% Ethylene glycol), Vapor Diffusion, Sitting Drop, temperature 298K