4NO0
Crystal structure of non-phosphorylated form of RQA_V phosphopeptide bound to HLA-A2 in complex with LILRB1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-21 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5417 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 117.300, 117.300, 96.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.325 - 2.700 |
R-factor | 0.2709 |
Rwork | 0.269 |
R-free | 0.30940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p7q |
RMSD bond length | 0.018 |
RMSD bond angle | 1.631 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.325 | 29.325 | 2.800 |
High resolution limit [Å] | 2.700 | 10.000 | 2.700 |
Rmerge | 0.126 | 0.042 | 0.816 |
Number of reflections | 21132 | 446 | 2171 |
<I/σ(I)> | 33.28 | 93.49 | 6.22 |
Completeness [%] | 98.9 | 93.7 | 99.5 |
Redundancy | 36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 18% PEG3350, 0.1 M HEPES, pH 7.4, 0.2 M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |