4NM0
Crystal structure of peptide inhibitor-free GSK-3/Axin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 81.303, 81.303, 280.829 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.048 - 2.500 |
R-factor | 0.1931 |
Rwork | 0.191 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nm7 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.626 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.050 | 2.600 | |
High resolution limit [Å] | 2.500 | 2.500 | |
Rmeas | 0.112 | 1.839 | 0.017 |
Rpim | 0.036 | 0.576 | 0.006 |
Total number of observations | 192347 | 21770 | 3969 |
Number of reflections | 20069 | ||
<I/σ(I)> | 18.4 | 1.2 | |
Completeness [%] | 100.0 | 99.9 | |
Redundancy | 9.6 | 9.9 | |
CC(1/2) | 0.999 | 0.726 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.5 | 277 | 10% PEG 35,000, 20mM Tris 7.5, 300mM NaCl, 5% glycerol, 20mM MgCl2, 400uM ATP, and 5mM DTT, MICRODIALYSIS, temperature 277K |