4NLN
Structure of human DNA polymerase beta complexed with nicked DNA containing a template 8BrG and incoming CTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.955, 79.075, 54.912 |
Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
Resolution | 52.667 - 2.261 |
R-factor | 0.2137 |
Rwork | 0.210 |
R-free | 0.27320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.842 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.667 | |
High resolution limit [Å] | 2.261 | 2.261 |
Number of reflections | 20822 | |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 14-23% PEG3400, 350 mM sodium acetate, 50 mM imidazole, pH 7.5, VAPOR DIFFUSION, SITTING DROP |