4NLM

1.18 Angstrom resolution crystal structure of uncharacterized protein lmo1340 from Listeria monocytogenes EGD-e

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	49.224, 69.990, 52.905
Unit cell angles	90.00, 95.61, 90.00

Refinement procedure

Resolution	24.210 - 1.180
R-factor	0.14678
Rwork	0.145
R-free	0.18116
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.594
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MLPHARE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.200
High resolution limit [Å]	1.180	1.180
Rmerge	0.076	0.459
Number of reflections	112400
<I/σ(I)>	31.7	2.33
Completeness [%]	95.9	97.4
Redundancy	3.6	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	protein - 7.5 mg/mL in 10 mM Tris-HCl, pH 8.3, 0.5 M NaCl 5 mM BME, Crystallization - The PACT Suite (#D2: 0.1 M MMT buffer pH 5, 25 % (w/v) PEG1500, VAPOR DIFFUSION, SITTING DROP, temperature 295K