4NLA
Structure of the central NEAT domain, N2, of the listerial Hbp2 protein, apo form
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950, 0.9686, 1.5418 |
Spacegroup name | I 2 3 |
Unit cell lengths | 89.300, 89.300, 89.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.140 - 2.700 |
R-factor | 0.258 |
Rwork | 0.254 |
R-free | 0.29300 |
Structure solution method | MAD |
Starting model (for MR) | 3sz6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.020 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.140 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.052 | 0.471 |
Number of reflections | 3370 | |
<I/σ(I)> | 35.3 | 6.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21.4 | 22.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.4 | 298 | 100 mM sodium citrate tribasic dihydrate, 200 mM potassium sodium tartrate tetrahydrate, 2.0 M ammonium sulfate, pH 5.4, vapor diffusion, temperature 298K, EVAPORATION |