4NKT
Structure of Cid1 in complex with the UTP analog UMPNPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9394 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.770, 77.320, 82.110 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 19.838 - 1.900 |
| R-factor | 0.1801 |
| Rwork | 0.179 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ep7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.044 | 0.044 |
| Number of reflections | 52778 | |
| <I/σ(I)> | 27.41 | 11.19 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 291 | 0.1 M imidazole/MES, pH 6.1, 20% glycerol, 10% PEG4000, 126 mM halogens (sodium iodide, sodium bromide, sodium fluoride), 10 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
