4NKR
The Crystal structure of Bacillus subtilis MobB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Detector technology | DIFFRACTOMETER |
Collection date | 2013-10-17 |
Detector | PHILLIPS |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 225.528, 42.107, 93.620 |
Unit cell angles | 90.00, 100.99, 90.00 |
Refinement procedure
Resolution | 29.270 - 2.410 |
R-factor | 0.23146 |
Rwork | 0.227 |
R-free | 0.32077 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.568 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CCP4 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 31830 | |
Completeness [%] | 98.8 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 23% PEG 3350, 0.4M Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |