4NKO
Crystal structure of engineered anti-EE scFv antibody fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.586, 104.917, 284.413 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.200 - 3.496 |
| R-factor | 0.3102 |
| Rwork | 0.308 |
| R-free | 0.34410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ktr |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.249 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 3.620 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Number of reflections | 11089 | |
| <I/σ(I)> | 12 | 2.97 |
| Completeness [%] | 93.1 | 95.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298.15 | Crystals of 3D5/His_683 (8mg/ml in HBS) were grown at room temperature with 0.2M KI, 0.001M Guanidinium HCl, 18% PEG 8000. These crystals appeared in 2-3 days., VAPOR DIFFUSION, SITTING DROP, temperature 298.15K |
