4NKJ

Structure of influenza B virus hemagglutinin at membrane fusion pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	200
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.127
Spacegroup name	H 3 2
Unit cell lengths	48.180, 48.180, 354.895
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.758 - 2.454
R-factor	0.2556
Rwork	0.255
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	0.810
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.760	2.540
High resolution limit [Å]	2.450	2.450
Rmerge		0.210
Number of reflections	5617
<I/σ(I)>		5.21
Completeness [%]	89.4	94.96
Redundancy		4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1