4NKI
Crystal structure of a Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Detector technology | CCD |
Collection date | 2011-06-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 115.944, 115.944, 78.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.710 - 2.410 |
R-factor | 0.1998 |
Rwork | 0.197 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.709 |
High resolution limit [Å] | 2.410 |
Number of reflections | 20258 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | The crystals were grown using hanging-drop vapor diffusion at 20 C by mixing equal volumes of the purified protein and the crystallization condition of 0.2M lithium sulfate monohydrate and 20% (wt/vol) polyethylene glycol 3350. , VAPOR DIFFUSION, HANGING DROP, temperature 293K |