4NK7
Crystal Structure of the D. melanogaster Plk4 cryptic polo box (CPB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2012-12-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9394 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 132.207, 132.207, 134.247 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.875 - 3.233 |
| R-factor | 0.2426 |
| Rwork | 0.240 |
| R-free | 0.26420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g7n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.059 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.430 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Number of reflections | 11543 | |
| <I/σ(I)> | 0.97 | |
| Completeness [%] | 99.3 | 96.4 |
| Redundancy | 41.8 | 41.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 100 mM HEPES-NaOH (pH 7.0), 2.5 M NaCl, 2 mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
