4NK7
Crystal Structure of the D. melanogaster Plk4 cryptic polo box (CPB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2012-12-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9394 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 132.207, 132.207, 134.247 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.875 - 3.233 |
R-factor | 0.2426 |
Rwork | 0.240 |
R-free | 0.26420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g7n |
RMSD bond length | 0.006 |
RMSD bond angle | 1.059 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.430 |
High resolution limit [Å] | 3.230 | 3.230 |
Number of reflections | 11543 | |
<I/σ(I)> | 0.97 | |
Completeness [%] | 99.3 | 96.4 |
Redundancy | 41.8 | 41.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 100 mM HEPES-NaOH (pH 7.0), 2.5 M NaCl, 2 mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |