4NJP
Proteolysis inside the membrane is a rate-governed reaction not Driven by substrate affinity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2012-07-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.54180 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 110.620, 110.620, 127.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.890 - 2.400 |
| R-factor | 0.18955 |
| Rwork | 0.187 |
| R-free | 0.24383 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ic8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.752 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.890 | 2.210 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 10817 | |
| Completeness [%] | 99.5 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES pH7.5, 3M NaCl, 10% Glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
