4NJP
Proteolysis inside the membrane is a rate-governed reaction not Driven by substrate affinity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2012-07-02 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.54180 |
Spacegroup name | H 3 2 |
Unit cell lengths | 110.620, 110.620, 127.730 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.890 - 2.400 |
R-factor | 0.18955 |
Rwork | 0.187 |
R-free | 0.24383 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ic8 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.752 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.890 | 2.210 |
High resolution limit [Å] | 2.170 | 2.170 |
Number of reflections | 10817 | |
Completeness [%] | 99.5 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES pH7.5, 3M NaCl, 10% Glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 298K |