4NJ9
Crystal structure of Fab 8B10 in complex with MPTS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.133, 58.441, 212.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.900 - 1.950 |
| R-factor | 0.2169 |
| Rwork | 0.214 |
| R-free | 0.26411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.840 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.900 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.668 |
| Number of reflections | 35255 | |
| <I/σ(I)> | 21.5 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M zinc acetate, 18% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
