4NJ2
GCN4-p1 triple Val9, 23,30 to Ile mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-15 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 39.380, 36.664, 47.631 |
| Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
| Resolution | 46.050 - 2.200 |
| R-factor | 0.25284 |
| Rwork | 0.247 |
| R-free | 0.31334 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zta |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.291 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.050 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.094 |
| Number of reflections | 5443 |
| <I/σ(I)> | 9.7 |
| Completeness [%] | 99.5 |
| Redundancy | 2.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.15M sodium citrate tribasic dihydrate, 20% v/v 2-propanol, 15% w/v PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
