4NJ1
GCN4-p1 double Val9, 23 to Ile mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-10 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.391, 30.477, 27.929 |
| Unit cell angles | 90.00, 102.08, 90.00 |
Refinement procedure
| Resolution | 27.310 - 2.000 |
| R-factor | 0.2417 |
| Rwork | 0.241 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dmd |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.331 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.550 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.028 |
| Number of reflections | 4582 |
| <I/σ(I)> | 28.3 |
| Completeness [%] | 96.2 |
| Redundancy | 2.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1 M soduim aceteate, 0.1 M sodium citrate tribasic dihyrdrate, 20% w/v PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
