4NIQ
Crystal Structure of Vps4 MIT-Vfa1 MIM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2013-04-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.476, 56.711, 84.233 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.998 - 2.301 |
| R-factor | 0.2379 |
| Rwork | 0.236 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.399 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.044 | 0.023 | 0.336 |
| Number of reflections | 8662 | ||
| <I/σ(I)> | 14.7 | ||
| Completeness [%] | 99.9 | 98.8 | 100 |
| Redundancy | 7.1 | 6.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 26% PEG 4000, 10% (v/v) isopropanol, 0.1M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
