4NIK

Structure of human Gankyrin in complex to the single chain antibody F5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-01
Detector	ADSC QUANTUM 4r
Spacegroup name	P 32 2 1
Unit cell lengths	135.578, 135.578, 68.655
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.633 - 2.500
R-factor	0.1658
Rwork	0.164
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uoh 3kfu
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	25163
Completeness [%]	98.9	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	8% PEG8000, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K