4NIA
Satellite Tobacco Mosaic Virus Refined at room temperature to 1.8 A Resolution using NCS Restraints
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-02-28 |
| Detector | SDMS |
| Wavelength(s) | 1.54178 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 174.268, 191.774, 202.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.040 - 1.820 |
| R-factor | 0.124 |
| Rwork | 0.123 |
| R-free | 0.15600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a34 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.263 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 87.040 | 500.000 | 1.919 |
| High resolution limit [Å] | 1.820 | 5.747 | 1.820 |
| Rmerge | 0.091 | ||
| Number of reflections | 268316 | ||
| Completeness [%] | 89.5 | 100 | 75 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 296 | 9 crystals were grown in microgravity aboard IML-I mission of the U.S. Space Shuttle over 12 days using 20% ammonium sulfate as precipitant, pH 6.50, LIQUID DIFFUSION, temperature 296K |
