4NHR
Crystal structure of the outer membrane lipopolysaccharide transport protein LptE (RlpB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97890 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 87.670, 87.670, 104.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.003 - 2.340 |
R-factor | 0.2272 |
Rwork | 0.226 |
R-free | 0.24910 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.126 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.395 | 61.400 |
High resolution limit [Å] | 2.170 | 2.340 |
Number of reflections | 10493 | |
<I/σ(I)> | 19.3 | |
Completeness [%] | 100.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 100 mM Tris/HOAc pH 8.5, 200 mM KBr, 10% PEG 1000, 10% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
2 |