4NHL
Crystal structure of Tpa1p from Saccharomyces cerevisiae, termination and polyadenylation protein 1, in complex with N-oxalylglycine (NOG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-07 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.216, 67.251, 70.976 |
| Unit cell angles | 90.00, 105.08, 90.00 |
Refinement procedure
| Resolution | 29.700 - 2.840 |
| Rwork | 0.181 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kt7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.282 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.940 |
| High resolution limit [Å] | 2.840 | 6.100 | 2.840 |
| Rmerge | 0.178 | 0.078 | 0.680 |
| Number of reflections | 18332 | ||
| <I/σ(I)> | 5.6 | 14.9 | 2.06 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 3.7 | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.8 mM MnCl2, 1.1 mM OGA, 0.1 M succinic acid, 12% PEG 3350, vapor diffusion sitting drop, temperature 293K, pH 7.0, VAPOR DIFFUSION, SITTING DROP |
