4NHL
Crystal structure of Tpa1p from Saccharomyces cerevisiae, termination and polyadenylation protein 1, in complex with N-oxalylglycine (NOG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-07 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.216, 67.251, 70.976 |
Unit cell angles | 90.00, 105.08, 90.00 |
Refinement procedure
Resolution | 29.700 - 2.840 |
Rwork | 0.181 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kt7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.282 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.940 |
High resolution limit [Å] | 2.840 | 6.100 | 2.840 |
Rmerge | 0.178 | 0.078 | 0.680 |
Number of reflections | 18332 | ||
<I/σ(I)> | 5.6 | 14.9 | 2.06 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 3.7 | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.8 mM MnCl2, 1.1 mM OGA, 0.1 M succinic acid, 12% PEG 3350, vapor diffusion sitting drop, temperature 293K, pH 7.0, VAPOR DIFFUSION, SITTING DROP |