4NHI

Crystal structure of Hen egg-white lysozyme in Tris buffer at pH 7.5 with Magnesium formate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-23
Detector	ADSC QUANTUM 270
Spacegroup name	P 43 21 2
Unit cell lengths	76.594, 76.594, 37.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.300 - 1.340
R-factor	0.16967
Rwork	0.167
R-free	0.21821
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lyz
RMSD bond length	0.022
RMSD bond angle	2.040
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.360
High resolution limit [Å]	1.340	1.340
Rmerge	0.137	0.536
Number of reflections	349012
<I/σ(I)>	59.724	1.6
Completeness [%]	99.8	99.7
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	80mg/ml hen egg-white lysozyme, 100mM TrisHCl, 0.4M Mg-Formate(0.2~0.6 M), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K