4NHI
Crystal structure of Hen egg-white lysozyme in Tris buffer at pH 7.5 with Magnesium formate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-23 |
Detector | ADSC QUANTUM 270 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.594, 76.594, 37.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.300 - 1.340 |
R-factor | 0.16967 |
Rwork | 0.167 |
R-free | 0.21821 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2lyz |
RMSD bond length | 0.022 |
RMSD bond angle | 2.040 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.360 |
High resolution limit [Å] | 1.340 | 1.340 |
Rmerge | 0.137 | 0.536 |
Number of reflections | 349012 | |
<I/σ(I)> | 59.724 | 1.6 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 80mg/ml hen egg-white lysozyme, 100mM TrisHCl, 0.4M Mg-Formate(0.2~0.6 M), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |