4NHG
Crystal Structure of 2G12 IgG Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 246.250, 246.250, 657.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.746 - 8.001 |
| R-factor | 0.3597 |
| Rwork | 0.359 |
| R-free | 0.36920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.617 |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.800 |
| High resolution limit [Å] | 7.500 |
| Number of reflections | 12654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.2 M ammonium sulfate, 100 mM HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
