4NGG
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 85.897, 97.240, 106.766 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.421 - 2.600 |
R-factor | 0.1888 |
Rwork | 0.186 |
R-free | 0.23930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ngd |
RMSD bond length | 0.008 |
RMSD bond angle | 1.094 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.088 | 0.036 | 0.722 |
Number of reflections | 14296 | ||
<I/σ(I)> | 9.5 | ||
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 9.7 | 8.9 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 0.2 M sodium chloride, 0.1 M sodium/potassium phosphate, pH 6.0-6.4, 10% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |