4NG8
Dialyzed HEW lysozyme batch crystallized in 1.9 M CsCl and collected at 100 K.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-03 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.935, 78.935, 37.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.820 - 1.090 |
R-factor | 0.155 |
Rwork | 0.152 |
R-free | 0.17600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 193l |
RMSD bond length | 0.017 |
RMSD bond angle | 1.543 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.300 | 1.160 |
High resolution limit [Å] | 1.090 | 1.090 |
Number of reflections | 91904 | |
<I/σ(I)> | 15.55 | 3.47 |
Completeness [%] | 98.5 | 98.1 |
Redundancy | 3.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 293 | Dialyzed lysozyme, no buffer added, 1.9 M CsCl, pH 4.5, Batch crystallization, temperature 293K |